677. Fluorescence Detection: Steady State, Time Resolved and Imaging

Course Level:

Graduate

Lecture - Lab Hours:

(4-0)

Credit:

4

Course Description:

Fluorescence spectroscopy and confocal/multiphoton microscopy in research; intro of pharmacology, life science, and physical science students to fluorophores, anisotropy, ligand binding, energy transfer, cytometry, lifetime imaging, correlation spectroscopy, immunocytochemistry, and image analysis with an emphasis on instrumental/sample artifacts, fluorescence application, literature evaluation, and communication of rationales to other scientists.

Prerequisite/Corequisite:

Prerequisite: General chemistry and biology course.